BarWare: efficient software tools for barcoded single-cell genomics.

ABSTRACT:

Background

Barcode-based multiplexing methods can be used to increase throughput and reduce batch effects in large single-cell genomics studies. Despite advantages in flexibility of sample collection and scale, there are additional complications in the data deconvolution steps required to assign each cell to their originating samples.

Results

To meet computational needs for efficient sample deconvolution, we developed the tools BarCounter and BarMixer that compute barcode counts and deconvolute mixed single-cell data into sample-specific files, respectively. Together, these tools are implemented as the BarWare pipeline to support demultiplexing from large sequencing projects with many wells of hashed 10x Genomics scRNA-seq data.

Conclusions

BarWare is a modular set of

SUBMITTER: Swanson E 

PROVIDER: S-EPMC8962164 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature