A transgenic mouse embryonic stem cell line for puromycin selection of V0_V interneurons from heterogenous induced cultures.

ABSTRACT:

Background

Spinal interneurons (INs) relay sensory and motor control information between the brain and body. When this relay circuitry is disrupted from injury or disease, it is devastating to patients due to the lack of native recovery in central nervous system (CNS) tissues. Obtaining a purified population of INs is necessary to better understand their role in normal function and as potential therapies in CNS. The ventral V0 (V0_V) INs are excitatory neurons involved in locomotor circuits and are thus of interest for understanding normal and pathological spinal cord function. To achieve scalable amounts of V0_V INs, they can be derived from pluripotent sources, such as mouse embryonic stem cells (mESCs), but the resultant culture is heterogenous, obscuring the

SUBMITTER: Pardieck J 

PROVIDER: S-EPMC8962475 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature