CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest gastrointestinal cancers with a 5-year survival rate of less than 10%. Biomarkers for early PDAC detection are useful in treating patients with PDAC. Extracellular vesicles (EVs) are lipid-bound vesicles that are potential biomarkers of various diseases such as PDAC. In this study, we quantitatively measured the serum levels of EVs (CD63⁺-EVs) or platelet-derived EVs (CD41⁺- and CD61⁺-EVs) and evaluated their potential use as biomarkers of PDAC.

Methods

We measured the serum levels of CD63⁺-, CD41⁺-, CD61⁺-EVs using sandwich enzyme-linked immunosorbent assay based on Tim4 with specificity for phosphatidylserine on EVs in age- and sex-ma

SUBMITTER: Odaka H 

PROVIDER: S-EPMC8962497 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature