An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer.

ABSTRACT:

Background

Tumor cells tend to utilize glycolysis rather than aerobic respiration even under aerobic conditions. OVOL2, an inhibitory C2H2 zinc finger transcription factor, is a potential tumor suppressor in cancers. However, the association between OVOL2 and tumor energy metabolism is unknown.

Methods

Western blotting was used to determine the expression of OVOL2 in different non-small cell lung cancer (NSCLC) cell lines and mouse models. The metabolic parameters in NSCLC cells following overexpression or knockdown OVOL2 were examined. To define the mechanism by which OVOL2 regulates aerobic glycolysis, interacting protein of OVOl2 and downstream molecular events were identified by luciferase assay and co-immunoprecipitation. We documented the regulatory mechanism in mouse

SUBMITTER: Zhang R 

PROVIDER: S-EPMC8962559 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature