Proteomic Analysis of the Non-genetic Response to Cisplatin in Lung Cancer Cells.

ABSTRACT:

Background

Drug resistance is the main cause of therapy failure in advanced lung cancer. Although non-genetic mechanisms play important roles in tumor chemoresistance, drug-induced epigenetic reprogramming is still poorly understood.

Materials and methods

The A549 cell line was used to generate cells with non-genetic resistance to cisplatin (CDDP), namely A549/CDDP cells. Bioorthogonal non-canonical amino acid tagging (BONCAT) and mass spectrometry were used to identify proteins modulated by CDDP in A549 and A549/CDDP cells.

Results

Proteins related to proteostasis, telomere maintenance, cell adhesion, cytoskeletal remodeling, and cell redox homeostasis were found enriched in both cell lines upon CDDP exposure. On the other hand, proteins involved in drug response, me

SUBMITTER: DE Souza Dutra C 

PROVIDER: S-EPMC8962784 | biostudies-literature | 2021 Jul-Aug

REPOSITORIES: biostudies-literature