Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.

ABSTRACT: The development of efficient and sensitive tools for the detection of brain cancer in patients is of the utmost importance particularly because many of these tumours go undiagnosed until the disease has advanced and when treatment is less effective. Current strategies employ antibodies (Abs) to detect Glial Fibrillary Acid Protein (GFAP) in tissue samples, since GFAP is unique to the brain and not present in normal peripheral blood, and it relies on fluorescent reporters. Herein we describe a low cost, practical and general method for the labelling of proteins and antibodies with fluorescent carbon dots (CD) to generate diagnostic probes that are robust, photostable and applicable to the clinical setting. The two-step protocol relies on the conjugation of a dibenzocyclooctyne (DBCO)-functi

SUBMITTER: Ghirardello M 

PROVIDER: S-EPMC8962998 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature