SARS-CoV-2 Infection Induces Ferroptosis of Sinoatrial Node Pacemaker Cells.

ABSTRACT:

Background

Increasing evidence suggests that cardiac arrhythmias are frequent clinical features of coronavirus disease 2019 (COVID-19). Sinus node damage may lead to bradycardia. However, it is challenging to explore human sinoatrial node (SAN) pathophysiology due to difficulty in isolating and culturing human SAN cells. Embryonic stem cells (ESCs) can be a source to derive human SAN-like pacemaker cells for disease modeling.

Methods

We used both a hamster model and human ESC (hESC)-derived SAN-like pacemaker cells to explore the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on the pacemaker cells of the heart. In the hamster model, quantitative real-time polymerase chain reaction and immunostaining were used to detect viral RNA and protein

SUBMITTER: Han Y 

PROVIDER: S-EPMC8963443 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature