TET2-mediated epigenetic reprogramming of breast cancer cells impairs lysosome biogenesis.

ABSTRACT: Methylation and demethylation of cytosines in DNA are believed to act as keystones of cell-specific gene expression by controlling the chromatin structure and accessibility to transcription factors. Cancer cells have their own transcriptional programs, and we sought to alter such a cancer-specific program by enforcing expression of the catalytic domain (CD) of the methylcytosine dioxygenase TET2 in breast cancer cells. The TET2 CD decreased the tumorigenic potential of cancer cells through both activation and repression of a repertoire of genes that, interestingly, differed in part from the one observed upon treatment with the hypomethylating agent decitabine. In addition to promoting the establishment of an antiviral state, TET2 activated 5mC turnover at thousands of MYC-binding motifs an

SUBMITTER: Laurent A 

PROVIDER: S-EPMC8963717 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature