Histone H3 and H4 tails play an important role in nucleosome phase separation.

ABSTRACT: Chromatin organization and its dynamic regulation are crucial in governing the temporal and spatial accessibility of DNA for proper gene expression. Disordered chains of nucleosomes comprise the basis of eukaryotic chromatin, forming higher-level organization across a range of length scales. Models of chromatin organization involving phase separation driven by chromatin-associating proteins have been proposed. More recently, evidence has emerged that nucleosome arrays can phase separate in the absence of other protein factors, yet questions remain regarding the molecular basis of chromatin phase separation that governs this dynamic nuclear organization. Here, we break chromatin down into its most basic subunit, the nucleosome core particle, and investigate phase separation using turbidity

SUBMITTER: Hammonds EF 

PROVIDER: S-EPMC8963862 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature