Protective Mechanism of Leucine and Isoleucine against H₂O₂-Induced Oxidative Damage in Bovine Mammary Epithelial Cells.

ABSTRACT: Leucine and isoleucine possess antioxidative and anti-inflammatory properties. However, their underlying protective mechanisms against oxidative damage remain unknown. Therefore, in this study, the protective mechanism of leucine and isoleucine against H₂O₂-induced oxidative damage in a bovine mammary epithelial cell lines (MAC-T cells) were investigated. Briefly, MAC-T cells exposed or free to H₂O₂ were incubated with different combinations of leucine and isoleucine. The cellular relative proliferation rate and viability, oxidative stress indicators, and inflammatory factors were determined by specific commercial kits. The genes related to barrier functions was measured by real-time quantitative PCR. The protein expression differences were explo

SUBMITTER: Wu S 

PROVIDER: S-EPMC8964234 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature