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Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

ABSTRACT: Hypoxia causes neonatal neuronal damage. However, the underlying mechanism remains unclear. This study aimed to explore the changes in succinate levels and identify the mechanisms underlying their contribution to hypoxia-induced damage in newborn mice. The neonatal C57BL/6J mouse hypoxia model was used in our study. We evaluated the levels of succinate, iron, reactive oxygen species (ROS), and mitochondrial ROS, and assessed mitophagy, neuronal damage, and learning and memory function, after hypoxia treatment. The neonatal mice showed increased succinate levels in the early hypoxia stage, followed by increased levels of oxidative stress, iron stress, neuronal damage, and cognitive deficits. Succinate levels were significantly reduced following treatment with inhibitors of succinate dehydrogenase (SDH), purine nucleotide cycle (PNC), and malate/aspartate shuttle (MAS), with the corresponding attenuation of oxidative stress, iron stress, neuronal damage, and cognitive impairment. Reversal catalysis of SDH through fumarate from the PNC and MAS pathways might be involved in hypoxia-induced succinate accumulation. Succinate accumulation in the early period after hypoxia may crucially contribute to oxidative and iron stress. Relieving succinate accumulation at the early hypoxia stage could prevent neuronal damage and cognitive impairment in neonatal hypoxia.

SUBMITTER: Zhang M

PROVIDER: S-EPMC8964675 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

Zhang Mengdi M Cheng Yao Y Zhai Yujie Y Cui Yaru Y Zhang Wenshen W Sun Hongwei H Xin Wenyu W Zhou Ling L Gao Xue X Li Shucui S Sun Hongliu H

Cell death discovery 20220328 1

Hypoxia causes neonatal neuronal damage. However, the underlying mechanism remains unclear. This study aimed to explore the changes in succinate levels and identify the mechanisms underlying their contribution to hypoxia-induced damage in newborn mice. The neonatal C57BL/6J mouse hypoxia model was used in our study. We evaluated the levels of succinate, iron, reactive oxygen species (ROS), and mitochondrial ROS, and assessed mitophagy, neuronal damage, and learning and memory function, after hyp ...[more]

PMID: 35351859

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Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

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Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

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