In vitro ballooned hepatocytes can be produced by primary human hepatocytes and hepatic stellate cell sheets.

ABSTRACT: Despite the increasing prevalence of Nonalcoholic steatohepatitis (NASH) worldwide, there is no effective treatment available for this disease. "Ballooned hepatocyte" is a characteristic finding in NASH and is correlated with disease prognosis, but their mechanisms of action are poorly understood; furthermore, neither animal nor in vitro models of NASH have been able to adequately represent ballooned hepatocytes. Herein, we engineered cell sheets to develop a new in vitro model of ballooned hepatocytes. Primary human hepatocytes (PHH) and Hepatic stellate cells (HSC) were co-cultured to produce cell sheets, which were cultured in glucose and lipid containing medium, following which histological and functional analyses were performed. Histological findings showed hepatocyte ballooning, accu

SUBMITTER: Hasui N 

PROVIDER: S-EPMC8964766 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature