Global transcriptomic characterization of T cells in individuals with chronic HIV-1 infection.

ABSTRACT: To obtain a comprehensive scenario of T cell profiles and synergistic immune responses, we performed single-cell RNA sequencing (scRNA-seq) on the peripheral T cells of 14 individuals with chronic human immunodeficiency virus 1 (HIV-1) infection, including nine treatment-naive (TP) and eight antiretroviral therapy (ART) participants (of whom three were paired with TP cases), and compared the results with four healthy donors (HD). Through analyzing the transcriptional profiles of CD4⁺ and CD8⁺ T cells, coupled with assembled T cell receptor sequences, we observed the significant loss of naive T cells, prolonged inflammation, and increased response to interferon-α in TP individuals, which could be partially restored by ART. Interestingly, we revealed that CD4⁺

SUBMITTER: Wang XM 

PROVIDER: S-EPMC8964811 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature