Effect of sampling site on the diagnosis of canine parvovirus infection in dogs using polymerase chain reaction.

ABSTRACT:

Background

Accurate diagnosis is imperative in dogs with clinical signs of parvovirus infection (CPV-2).

Objectives

To assess quantitative real-time PCR (qRT-PCR) for the diagnosis of CPV-2 infection, and determine the optimal sampling site. Secondarily, to compare qRT-PCR with a point-of-care PCR kit (PCRun), and to assess sensitivity of serology for CPV diagnosis.

Animals

Sixty dogs with naturally acquired parvovirus infection, 44 unvaccinated puppies, of which 16 were followed after first and second vaccination, 15 adult dogs, of which 10 were followed also after a booster vaccine, and 9 dogs with distemper virus infection.

Methods

Prospective study. Samples from the rectum, blood, and pharynx were obtained for PCR.

Results

All dogs with a clinical di

SUBMITTER: Segev G 

PROVIDER: S-EPMC8965247 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature