Effect of early or late blood sampling on thyrotropin releasing hormone stimulation test results in horses.

ABSTRACT:

Background

Diagnosis of pituitary pars intermedia dysfunction (PPID) using the thyrotropin-releasing hormone (TRH) stimulation test requires blood collection 10 minutes after TRH injection; it is unknown if small differences in timing affect test results.

Objective

To determine whether early or late sampling results in a significant (≥10%) difference in plasma adrenocorticotropic hormone (ACTH) concentration compared to standard 10-minute sampling.

Animals

Twenty-four healthy adult horses with unknown PPID status.

Methods

In this prospective study, subjects underwent a single TRH stimulation test, with blood collected exactly 9 minutes (early), 10 minutes (standard), and 11 minutes (late) after injection. ACTH was measured by chemiluminescent immunoassay. Two a

SUBMITTER: Thane K 

PROVIDER: S-EPMC8965261 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature