Structure-based design and construction of a synthetic phage display nanobody library.

ABSTRACT:

Objective

To design and construct a new synthetic nanobody library using a structure-based approach that seeks to maintain high protein stability and increase the number of functional variants within the combinatorial space of mutations.

Results

Synthetic nanobody (Nb) libraries are emerging as an attractive alternative to animal immunization for the selection of stable, high affinity Nbs. Two key features define a synthetic Nb library: framework selection and CDR design. We selected the universal VHH framework from the cAbBCII10 Nb. CDR1 and CDR2 were designed with the same fixed length as in cAbBCII10, while for CDR3 we chose a 14-long loop, which creates a convex binding site topology. Based on the analysis of the cAbBCII10 crystal structure, we carefully selected the pos

SUBMITTER: Moreno E 

PROVIDER: S-EPMC8966178 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature