A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging.

ABSTRACT:

Background

Tissue-clearing techniques have recently been developed to make tissues transparent for three-dimensional (3D) imaging at different scales, including single-cell resolution. However, current tissue-clearing workflows have several disadvantages, including complex protocols, time-consuming application, and fluorescence quenching. Additionally, they can be used mainly for clearing larger-volume samples, preventing wide and easy applicability in conventional experimental approaches. In this study, we aimed to develop a versatile, fast, and convenient method for clearing thin and semi-thick samples, which can be used for three-dimensional imaging of experimental or even clinical samples.

Results

We developed an alkaline solution (AKS) containing a combination of 2,2'-t

SUBMITTER: Shan QH 

PROVIDER: S-EPMC8966190 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature