Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means.

ABSTRACT:

Background

Type I interferons are widely used in research applications and as biotherapeutics. Current assays used to measure interferon concentrations, such as plaque reduction assays and ELISA, are expensive, technically challenging, and may take days to provide results. We sought to develop a robust and rapid assay to determine interferon concentrations produced from transiently transfected cell cultures.

Method

Indirect quantification of recombinant interferon was evaluated using a novel bi-cistronic construct encoding the Foot-and-mouth disease virus 2A translational interrupter sequence to yield equimolar expression of Gaussia princeps luciferase and porcine interferon α. Direct quantification was evaluated by expression of a novel fusion protein comprised of Gaussia p

SUBMITTER: Puckette M 

PROVIDER: S-EPMC8966355 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature