In-Cell Labeling and Mass Spectrometry for Systems-Level Structural Biology.

ABSTRACT: Biological systems have evolved to utilize proteins to accomplish nearly all functional roles needed to sustain life. A majority of biological functions occur within the crowded environment inside cells and subcellular compartments where proteins exist in a densely packed complex network of protein-protein interactions. The structural biology field has experienced a renaissance with recent advances in crystallography, NMR, and CryoEM that now produce stunning models of large and complex structures previously unimaginable. Nevertheless, measurements of such structural detail within cellular environments remain elusive. This review will highlight how advances in mass spectrometry, chemical labeling, and informatics capabilities are merging to provide structural insights on proteins, complexe

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC8966414 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature