Intramolecular hydrogen transfer in DNA induced by site-selective resonant core excitation.

ABSTRACT: We present experimental evidence for soft X-ray induced intramolecular hydrogen transfer in the protonated synthetic tri-oligonucleotide d(^FUAG) in the gas-phase (^FU: fluorouracil). The trinucleotide cations were stored in a cryogenic ion trap and exposed to monochromatic synchrotron radiation. Photoionization and photofragmentation product ion yields were recorded as a function of photon energy. Predominanly glycosidic bond cleavage leading to formation of nucleobase-related fragments is observed. In most cases, glycosidic bond cleavage is accompanied by single or double hydrogen transfer. The combination of absorption-site-sensitive soft X-ray spectroscopy with fragment specific mass spectrometry allows to directly relate X-ray absorption site and fragmentati

SUBMITTER: Wang X 

PROVIDER: S-EPMC8966622 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature