Bachu Mushroom Polysaccharide Alleviates Colonic Injury by Modulating the Gut Microbiota.
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ABSTRACT: Objective
This study was to define the protective effect of purified Helvella leucopus polysaccharide (p-HLP) against dextran sulfate sodium- (DSS-) induced colitis.Methods
The novel p-HLP was isolated from Bachu mushroom through hot water extraction, ethanol precipitation, and column chromatography. Then, we evaluated the potential effects of p-HLP on colonic histopathology, inflammation, and microbiota composition in DSS-induced colitis mice.Results
p-HLP was a homopolysaccharide with an average molecular weight of 39.14 × 108 Da. Functionally, p-HLP significantly attenuated DSS-induced body weight loss and colon shortening. The histological score of the colon lesion was significantly decreased upon p-HLP treatment. Also, p-HLP treatment led to decreased expression of proinflammatory cytokines and mediators (IL-6, IL-1β and TNF-α, and COX-2 and iNOS) and increased expression of anti-inflammatory cytokine (IL-10) in the colon tissues. Illumina MiSeq sequencing revealed that p-HLP modulated the composition of the gut microbiota.Conclusion
p-HLP is a potent regulator that protects the lesions from DSS-induced colitis.
SUBMITTER: Abdureyim Z
PROVIDER: S-EPMC8966746 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Publications
Bachu Mushroom Polysaccharide Alleviates Colonic Injury by Modulating the Gut Microbiota.
Abdureyim Zumrat Z Wang Lei L Tao Qing Q Xu Jing J Yimiti Delixiati D Gao Qian Q
Computational and mathematical methods in medicine 20220314
<h4>Objective</h4>This study was to define the protective effect of purified <i>Helvella leucopus</i> polysaccharide (p-HLP) against dextran sulfate sodium- (DSS-) induced colitis.<h4>Methods</h4>The novel p-HLP was isolated from Bachu mushroom through hot water extraction, ethanol precipitation, and column chromatography. Then, we evaluated the potential effects of p-HLP on colonic histopathology, inflammation, and microbiota composition in DSS-induced colitis mice.<h4>Results</h4>p-HLP was a h ...[more]
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