Toward Understanding the Alginate Catabolism in Microbulbifer sp. ALW1 by Proteomics Profiling.

ABSTRACT: The bacterial strain of Microbulbifer sp. ALW1 has demonstrated visible ability of degrading the cell wall of Laminaria japonica, and biochemical characterization has been performed on some individual enzymes to elucidate its genetic basis. However, it still remains elusive how strain ALW1 successfully breaks down the major cell wall component alginate polysaccharide and colonizes on its marine host. In this study, a mass spectrometry-based quantitative analysis of the extracellular and intracellular proteomes was introduced to elucidate the alginate degradation pathway in ALW1 strain. Mass spectrometry and biochemical assays indicated that strain ALW1 could effectively degrade alginate polysaccharide into disaccharides and trisaccharides within 12 h. Proteome analysis identi

SUBMITTER: Li Z 

PROVIDER: S-EPMC8967155 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature