EIF4A2 targets developmental potency and histone H3.3 transcripts for translational control of stem cell pluripotency.

ABSTRACT: Translational control has emerged as a fundamental regulatory layer of proteome complexity that governs cellular identity and functions. As initiation is the rate-limiting step of translation, we carried out an RNA interference screen for key translation initiation factors required to maintain embryonic stem cell (ESC) identity. We identified eukaryotic translation initiation factor 4A2 (eIF4A2) and defined its mechanistic action through ribosomal protein S26-independent and -dependent ribosomes in translation initiation activation of messenger RNAs (mRNAs) encoding pluripotency factors and the histone variant H3.3 with demonstrated roles in maintaining stem cell pluripotency. eIF4A2 also mediates translation initiation activation of Ddx6, which acts together with eIF4A2 to restrict the to

SUBMITTER: Li D 

PROVIDER: S-EPMC8967233 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature