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Formation of cellular close-ended tunneling nanotubes through mechanical deformation.

ABSTRACT: Membrane nanotubes or tunneling nanotubes (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust for hours, is formed. Here, we found that a TNT develops from a double filopodial bridge (DFB) created by the physical contact of two filopodia through helical deformation of the DFB. The transition of a DFB to a close-ended TNT is most likely triggered by disruption of the adhesion of two filopodia by mechanical energy accumulated in a twisted DFB when one of the DFB ends is firmly attached through intercellular cadherin-cadherin interactions. These studies pinpoint the mechanistic questions about TNTs and elucidate a formation mechanism.

SUBMITTER: Chang M

PROVIDER: S-EPMC8967236 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Formation of cellular close-ended tunneling nanotubes through mechanical deformation.

Chang Minhyeok M Lee O-Chul OC Bu Gayun G Oh Jaeho J Yunn Na-Oh NO Ryu Sung Ho SH Kwon Hyung-Bae HB Kolomeisky Anatoly B AB Shim Sang-Hee SH Doh Junsang J Jeon Jae-Hyung JH Lee Jong-Bong JB

Science advances 20220330 13

Membrane nanotubes or tunneling nanotubes (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust for hours, is formed. Here, we found that a TNT develops from a double filopodial bridge (DFB) created by the physical contact of two filopodia through helical deformation of the DFB. The transition of a DFB to a ...[more]

PMID: 35353579

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