A proteomics approach identifies novel resident zebrafish Balbiani body proteins Cirbpa and Cirbpb.

ABSTRACT: The Balbiani body (Bb) is the first marker of polarity in vertebrate oocytes. The Bb is a conserved structure found in diverse animals including insects, fish, amphibians, and mammals. During early zebrafish oogenesis, the Bb assembles as a transient aggregate of mRNA, proteins, and membrane-bound organelles at the presumptive vegetal side of the oocyte. As the early oocyte develops, the Bb appears to grow slowly, until at the end of stage I of oogenesis it disassembles and deposits its cargo of localized mRNAs and proteins. In fish and frogs, this cargo includes the germ plasm as well as gene products required to specify dorsal tissues of the future embryo. We demonstrate that the Bb is a stable, solid structure that forms a size exclusion barrier similar to other biological hydrogels. De

SUBMITTER: Jamieson-Lucy AH 

PROVIDER: S-EPMC8967276 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature