Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts.

ABSTRACT:

Purpose

The present study aimed to examine the correlations of the time interval from trophectoderm (TE) biopsy to vitrification with the blastocyst survival rate and blastocyst outgrowth ability.

Methods

A total of 1,202 mouse blastocysts were randomly divided into control (non-biopsy) and TE biopsy groups. The biopsied blastocysts were vitrified at various time points. The survival rate after warming, blastocyst adhesion rate, and outgrowth area was investigated. Several biopsied blastocysts were cultured in a time-lapse incubator, and the time required for re-expansion was measured.

Results

Blastocyst survival rates after warming and blastocyst adhesion rates were comparable between the control and biopsy groups. The area of trophoblast outgrowth in the 1-h biopsy

SUBMITTER: Miki T 

PROVIDER: S-EPMC8967302 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature