A gene network of uterine luminal epithelium organizes mouse blastocyst implantation.

ABSTRACT:

Purpose

The receptive endometrium is critical for blastocyst implantation. In mice, after blastocysts enter the uterine cavities on day 4 of pregnancy (day 1 = vaginal plug), blastocyst attachment is completed within 24 h, accompanied by dynamic interactions between the uterine luminal epithelium and the blastocysts. Any failures in this process compromise subsequent pregnancy outcomes. Here, we performed comprehensive analyses of gene expression at the luminal epithelium in the peri-implantation period.

Methods

RNA-seq combined with laser microdissection (LMD) was used to reveal unique gene expression kinetics in the epithelium.

Results

We found that the prereceptive epithelium on day 3 specifically expresses cell cycle-related genes. In addition, days 3 and 4 epithe

SUBMITTER: Aikawa S 

PROVIDER: S-EPMC8967306 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature