A Comprehensive 2D-LC/MS Online Platform for Screening of Acetylcholinesterase Inhibitors.

ABSTRACT: The continuous interest in discovering new bioactive molecules derived from natural products (NP) has stimulated the development of improved screening assays to help overcome challenges in NP-based drug discovery. Here, we describe a unique platform for the online screening of acetylcholinesterase inhibitors without the need for pre-treating the sample. In the current study, we have demonstrated the ability to combine reversed-phase separation with a capillary immobilized enzyme reactor (cIMER) in two-dimensional liquid chromatography system coupled with mass spectrometry detection. We systematically investigated the effects of method parameters that are of practical significance and are known to affect the enzyme assay and interfere in the analysis such as: bioreactor dimensions, loop siz

SUBMITTER: Seidl C 

PROVIDER: S-EPMC8967351 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature