The bacteriology of pleural infection (TORPIDS): an exploratory metagenomics analysis through next generation sequencing.

ABSTRACT:

Background

Pleural infection is a common and severe disease with high morbidity and mortality worldwide. The knowledge of pleural infection bacteriology remains incomplete, as pathogen detection methods based on culture have insufficient sensitivity and are biased to selected microbes. We designed a study with the aim to discover and investigate the total microbiome of pleural infection and assess the correlation between bacterial patterns and 1-year survival of patients.

Methods

We assessed 243 pleural fluid samples from the PILOT study, a prospective observational study on pleural infection, with 16S rRNA next generation sequencing. 20 pleural fluid samples from patients with pleural effusion due to a non-infectious cause and ten PCR-grade water samples were used as contro

SUBMITTER: Kanellakis NI 

PROVIDER: S-EPMC8967721 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature