Acoustic levitation and rotation of thin films and their application for room temperature protein crystallography.

ABSTRACT: Acoustic levitation has attracted attention in terms of chemical and biochemical analysis in combination with various analytical methods because of its unique container-less environment for samples that is not reliant on specific material characteristics. However, loading samples with very high viscosity is difficult. To expand the scope, we propose the use of polymer thin films as sample holders, whereby the sample is dispensed on a film that is subsequently loaded onto an acoustic levitator. When applied for protein crystallography experiments, rotation controllability and positional stability are important prerequisites. We therefore study the acoustic levitation and rotation of thin films with an aspect ratio (the diameter-to-thickness ratio) of 80-240, which is an order of magnitude l

SUBMITTER: Kepa MW 

PROVIDER: S-EPMC8967846 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature