Integrating protein copy numbers with interaction networks to quantify stoichiometry in clathrin-mediated endocytosis.

ABSTRACT: Proteins that drive processes like clathrin-mediated endocytosis (CME) are expressed at copy numbers within a cell and across cell types varying from hundreds (e.g. auxilin) to millions (e.g. clathrin). These variations contain important information about function, but without integration with the interaction network, they cannot capture how supply and demand for each protein depends on binding to shared and distinct partners. Here we construct the interface-resolved network of 82 proteins involved in CME and establish a metric, a stoichiometric balance ratio (SBR), that quantifies whether each protein in the network has an abundance that is sub- or super-stoichiometric dependent on the global competition for binding. We find that highly abundant proteins (like clathrin) are super-stoichio

SUBMITTER: Duan D 

PROVIDER: S-EPMC8967901 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature