Development of a single-cell atlas for woodland strawberry (Fragaria vesca) leaves during early Botrytis cinerea infection using single cell RNA-seq.

ABSTRACT: Pathogen invasion leads to fast, local-to-systemic signal transduction that initiates plant defense responses. Despite tremendous progress in past decades, aspects of this process remain unknown, such as which cell types respond first and how signals are transferred among cell types. Here, we used single-cell RNA-seq of more than 50 000 single cells to document the gene expression landscape in leaves of woodland strawberry during infection by Botrytis cinerea and identify major cell types. We constructed a single-cell atlas and characterized the distinct gene expression patterns of hydathode, epidermal, and mesophyll cells during the incubation period of B. cinerea infection. Pseudotime trajectory analysis revealed signals of the transition from normal functioning to defense response in ep

SUBMITTER: Bai Y 

PROVIDER: S-EPMC8969069 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature