Single molecule iSCAT imaging reveals a fast, energy efficient search mode for the DNA repair protein UvrA.

ABSTRACT: Exposure to UV radiation results in numerous DNA lesions, which threaten genome integrity. The nucleotide excision DNA repair pathway detects and repairs a range of such UV-induced DNA lesions. In bacteria, initial damage detection and verification is carried out by two proteins: UvrA and UvrB. Despite decades of study, the process of how these proteins locate damage remains unclear. Here we use high-speed interferometric scattering (iSCAT) microscopy, in combination with a surface-bound-DNA assay, to investigate early damage detection by UvrA. We have discovered that UvrA interacts with DNA in two phases; a slow phase (∼1.3 s^-1) that correlates with an ATP-consuming state previously identified, and a second, much faster search mode. These faster interactions persist for ∼130 ms

SUBMITTER: Charman RJ 

PROVIDER: S-EPMC8969456 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature