Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.

ABSTRACT:

Objective

To clarify the function and mechanisms of sevoflurane (Sev) on ferroptosis in glioma cells.

Methods

Different concentrations of Sev were used to treat glioma cells U87 and U251. Ferroptosis inducer Erastin was used to incubate glioma cells combined with Sev and ATF4 siRNA transfection treatment. CCK-8 assay and colorimetric assay were performed to analyze cell viability and Fe⁺ concentration, respectively. The releases of reactive oxygen species (ROS) were determined by flow cytometry analysis. Transcriptional sequencing was used to screen the differential genes affected by Sev in U251 cells. The mRNA and protein expression of ferroptosis-associated genes was detected by qRT-PCR and Western blotting.

Results

Sev could suppress cell viability, incr

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8969566 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature