Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions In Situ.

ABSTRACT: Tissue microenvironment properties like blood flow, extracellular matrix, or proximity to immune-infiltrate are important regulators of cell biology. However, methods to study regional protein expression in the native tissue environment are limited. To address this need, we developed a novel approach to visualize, purify, and measure proteins in situ using automated spatially targeted optical microproteomics (AutoSTOMP). Here, we report custom codes to specify regions of heterogeneity in a tissue section and UV-biotinylate proteins within those regions. We have developed liquid chromatography-mass spectrometry (LC-MS)/MS-compatible biochemistry to purify those proteins and label-free quantification methodology to determine protein enrichment in target cell types or structures relati

SUBMITTER: Yin B 

PROVIDER: S-EPMC8969901 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature