α-Parvin Defines a Specific Integrin Adhesome to Maintain the Glomerular Filtration Barrier.

ABSTRACT:

Background

The cell-matrix adhesion between podocytes and the glomerular basement membrane is essential for the integrity of the kidney's filtration barrier. Despite increasing knowledge about the complexity of integrin adhesion complexes, an understanding of the regulation of these protein complexes in glomerular disease remains elusive.

Methods

We mapped the in vivo composition of the podocyte integrin adhesome. In addition, we analyzed conditional knockout mice targeting a gene (Parva) that encodes an actin-binding protein (α-parvin), and murine disease models. To evaluate podocytes in vivo, we used super-resolution microscopy, electron microscopy, multiplex immunofluorescence microscopy, and RNA sequencing. We performed functional analysis of CRISPR/

SUBMITTER: Rogg M 

PROVIDER: S-EPMC8970443 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature