Claudin-10a Deficiency Shifts Proximal Tubular Cl^- Permeability to Cation Selectivity via Claudin-2 Redistribution.

ABSTRACT:

Background

The tight junction proteins claudin-2 and claudin-10a form paracellular cation and anion channels, respectively, and are expressed in the proximal tubule. However, the physiologic role of claudin-10a in the kidney has been unclear.

Methods

To investigate the physiologic role of claudin-10a, we generated claudin-10a-deficient mice, confirmed successful knockout by Southern blot, Western blot, and immunofluorescence staining, and analyzed urine and serum of knockout and wild-type animals. We also used electrophysiologic studies to investigate the functionality of isolated proximal tubules, and studied compensatory regulation by pharmacologic intervention, RNA sequencing analysis, Western blot, immunofluorescence staining, and respirometry.

Results

Mice defici

SUBMITTER: Breiderhoff T 

PROVIDER: S-EPMC8970455 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature