Assessment of genetic variation in Apis mellifera jemenitica (Hymenoptera: Apidae) based on mitochondrial Cytochrome Oxidase Subunit II and III.

ABSTRACT: Morphometric and genetic characterization of many Apis mellifera subspecies are well-documented. A. m. jemenetica occurs naturally in Africa and Asia. In this study, genetic variation of mitochondrial Cytochrome Oxidase II (COII) and III (COIII) were analysed in 133 specimens of the endemic honeybee colonies within Saudi Arabia. The COII gene sequence length was 684 bp comprising nine synonymous (1.3%) and two non-synonymous single nucleotide polymorphisms (SNPs) (0.87%). Five variants of COII were not previously documented, one variant (MT755968) showed an extra restriction site when subjected to type II restriction endonuclease from Arthrobacter protophormiae (Apol) or to Haemophilus influenzae Rf (Hinf1). Changes in COII sequence separated samples into three haplogroups. Whereas, COIII

SUBMITTER: Alattal Y 

PROVIDER: S-EPMC8970473 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature