Ultrasonication of Milk Decreases the Content of Exosomes and MicroRNAs in an Exosome-Defined Rodent Diet.

ABSTRACT:

Background

Bovine milk exosomes (BMEs) harbor regulatory proteins, lipids, and microRNAs. Consumption of an exosome- and RNA-depleted (ERD) diet elicited phenotypes compared with controls fed an exosome- and RNA-sufficient (ERS) diet in mice. All other ingredients were identical in the diets. ERD and ERS diets were prepared by substituting ultrasonicated and nonultrasonicated milk, respectively, for casein in the AIN-93G formulation.

Objectives

The objective of this study was to assess the effect of ultrasonication of milk on exosome content and bioavailability, and cargo content.

Methods

Bovine milk was ultrasonicated and exosomes were isolated by ultracentrifugation [ultrasonicated exosomes (USEs)]; controls were not ultrasonicated [nonultrasonicated exosomes (NSEs)

SUBMITTER: Sukreet S 

PROVIDER: S-EPMC8970979 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature