Basal oxidation of conserved cysteines modulates cardiac titin stiffness and dynamics.

ABSTRACT: Titin, as the main protein responsible for the passive stiffness of the sarcomere, plays a key role in diastolic function and is a determinant factor in the etiology of heart disease. Titin stiffness depends on unfolding and folding transitions of immunoglobulin-like (Ig) domains of the I-band, and recent studies have shown that oxidative modifications of cryptic cysteines belonging to these Ig domains modulate their mechanical properties in vitro. However, the relevance of this mode of titin mechanical modulation in vivo remains largely unknown. Here, we describe the high evolutionary conservation of titin mechanical cysteines and show that they are remarkably oxidized in murine cardiac tissue. Mass spectrometry analyses indicate a similar landscape of basal oxidation in murine and human

SUBMITTER: Herrero-Galan E 

PROVIDER: S-EPMC8971355 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature