Targeting the tumor mutanome for personalized vaccination in a TMB low non-small cell lung cancer.

ABSTRACT:

Background

Cancer is characterized by an accumulation of somatic mutations, of which a significant subset can generate cancer-specific neoepitopes that are recognized by autologous T cells. Such neoepitopes are emerging as important targets for cancer immunotherapy, including personalized cancer vaccination strategies.

Methods

We used whole-exome and RNA sequencing analysis to identify potential neoantigens for a patient with non-small cell lung cancer. Thereafter, we assessed the autologous T-cell reactivity to the candidate neoantigens using a long peptide approach in a cultured interferon gamma ELISpot and tracked the neoantigen-specific T-cells in the tumor by T-cell receptor (TCR) sequencing. In parallel, identified gene variants were incorporated into a Modified Vaccin

SUBMITTER: McCann K 

PROVIDER: S-EPMC8971766 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature