Spatial proteomics reveals subcellular reorganization in human keratinocytes exposed to UVA light.

ABSTRACT: The effects of UV light on the skin have been extensively investigated. However, systematic information about how the exposure to ultraviolet-A (UVA) light, the least energetic but the most abundant UV radiation reaching the Earth, shapes the subcellular organization of proteins is lacking. Using subcellular fractionation, mass-spectrometry-based proteomics, machine learning algorithms, immunofluorescence, and functional assays, we mapped the subcellular reorganization of the proteome of human keratinocytes in response to UVA light. Our workflow quantified and assigned subcellular localization for over 1,600 proteins, of which about 200 were found to redistribute upon UVA exposure. Reorganization of the proteome affected modulators of signaling pathways, cellular metabolism, and DNA damage

SUBMITTER: Valerio HP 

PROVIDER: S-EPMC8971936 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature