Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live.

ABSTRACT: Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro Live^TM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

SUBMITTER: Slangen PLG 

PROVIDER: S-EPMC8971986 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature