Establishment of patient-derived organoid models of lower-grade glioma.

ABSTRACT:

Background

Historically, creating patient-derived models of lower-grade glioma (LGG) has been challenging, contributing to few experimental platforms that support laboratory-based investigations of this disease. Although organoid modeling approaches have recently been employed to create in vitro models of high-grade glioma (HGG), it is unknown whether this approach can be successfully applied to LGG.

Methods

In this study, we developed an optimized protocol for the establishment of organoids from LGG primary tissue samples by utilizing physiologic (5%) oxygenation conditions and employed it to produce the first known suite of these models. To assess their fidelity, we surveyed key biological features of patient-derived organoids using metabolic, genomic, histologic, and line

SUBMITTER: Abdullah KG 

PROVIDER: S-EPMC8972292 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature