Calculation and Visualization of Binding Equilibria in Protein Studies.

ABSTRACT: A set of simulation applets has been developed for visualizing the behavior of the association and dissociation reactions in protein studies. These reactions are simple equilibrium reactions, and the equilibrium constants, most often dissociation constant K _D, are useful measures of affinity. Equilibria, even in simple systems, may not behave intuitively, which can cause misconceptions and mistakes. These applets can be utilized for planning experiments, for verifying experimental results, and for visualization of the equilibria in education. The considered reactions include protein homodimerization, ligand binding to a receptor (or heterodimerization), and competitive ligand binding. The latter one can be considered as either a ligand binding to two receptors or a bindin

SUBMITTER: Paakkonen J 

PROVIDER: S-EPMC8973030 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature