Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain.

ABSTRACT: A main impediment to effective development of new therapeutics for central nervous system disorders, and for the in vivo testing of biological hypotheses in the brain, is the ability to rapidly measure the effect of novel agents and treatment combinations on the pathophysiology of native brain tissue. We have developed a miniaturized implantable microdevice (IMD) platform, optimized for direct stereotactic insertion into the brain, which enables the simultaneous measurement of multiple drug effects on the native brain tissue in situ. The IMD contains individual reservoirs which release microdoses of single agents or combinations into confined regions of the brain, with subsequent spatial analysis of phenotypic, transcriptomic or metabolomic effects. Using murine models of Alz

SUBMITTER: Kim J 

PROVIDER: S-EPMC8973214 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature