Context-dependent DNA polymerization effects can masquerade as DNA modification signals.

ABSTRACT:

Background

Single molecule measurements of DNA polymerization kinetics provide a sensitive means to detect both secondary structures in DNA and deviations from primary chemical structure as a result of modified bases. In one approach to such analysis, deviations can be inferred by monitoring the behavior of DNA polymerase using single-molecule, real-time sequencing with zero-mode waveguide. This approach uses a Single Molecule Real Time (SMRT)-sequencing measurement of time between fluorescence pulse signals from consecutive nucleosides incorporated during DNA replication, called the interpulse duration (IPD).

Results

In this paper we present an analysis of loci with high IPDs in two genomes, a bacterial genome (E. coli) and a eukaryotic genome (C. elegans). To distinguish t

SUBMITTER: Takahashi Y 

PROVIDER: S-EPMC8973881 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature