Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

ABSTRACT:

Purpose

To explore the function and regulatory mechanism of IFITM3 in mouse neural retinal progenitor cells (mNRPCs), which was found to be very important not only in the development of the retina in embryos but also in NRPCs after birth.

Methods

Published single-cell sequencing data were used to analyze IFITM3 expression in mNRPCs. RNA interference was used to knock down the expression of IFITM3. CCK-8 assays were used to analyze cell viability. RNA-seq was used to assess mRNA expression, as confirmed by real-time quantitative PCR, and immunofluorescence assays and western blots were used to validate the levels of relative proteins, and autophagy flux assay. Lysosomal trackers were used to track the organelle changes.

Results

The results of single-cell sequencing dat

SUBMITTER: Jin C 

PROVIDER: S-EPMC8973999 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature