A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver.

ABSTRACT:

Background

Understanding the contribution of gene function in distinct organ systems to the pathogenesis of human diseases in biomedical research requires modifying gene expression through the generation of gain- and loss-of-function phenotypes in model organisms, for instance, the mouse. However, methods to modify both germline and somatic genomes have important limitations that prevent easy, strong, and stable expression of transgenes. For instance, while the liver is remarkably easy to target, nucleic acids introduced to modify the genome of hepatocytes are rapidly lost, or the transgene expression they mediate becomes inhibited due to the action of effector pathways for the elimination of exogenous DNA. Novel methods are required to overcome these challenges, and here we develo

SUBMITTER: Kopasz AG 

PROVIDER: S-EPMC8974095 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature