DEPTH2: an mRNA-based algorithm to evaluate intratumor heterogeneity without reference to normal controls.

ABSTRACT:

Background

Intratumor heterogeneity (ITH) is associated with tumor progression, unfavorable prognosis, immunosuppression, genomic instability, and therapeutic resistance. Thus, evaluation of ITH levels is valuable in cancer diagnosis and treatment.

Methods

We proposed a new mRNA-based ITH evaluation algorithm (DEPTH2) without reference to normal controls. DEPTH2 evaluates ITH levels based on the standard deviations of absolute z-scored transcriptome levels in tumors, reflecting the asynchronous level of transcriptome alterations relative to the central tendency in a tumor.

Results

By analyzing 33 TCGA cancer types, we demonstrated that DEPTH2 ITH was effective in measuring ITH for its significant associations with tumor progression, unfavorable prognosis, genomic inst

SUBMITTER: Song D 

PROVIDER: S-EPMC8974098 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature